![]() If there is too much background then the quality of the stain is poor and results in images that are difficult to analyze. The importance of antibody dilution is obvious in staining applications because there is significant background staining when the antibody concentration is too high. ![]() Why are serial dilutions of antibodies necessary? In microbiology, serial dilutions (log dilutions) are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate. What is the purpose of conducting serial dilutions? In biochemistry, serial dilution is used to obtain the desired concentration of reagents and chemicals from a higher concentration. Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies. What is the importance of serial dilution in serology?
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